Hypolipidaemic Potentials of Vernonia amygdalina (Bitter Leaf) in male albino rats fed high-sucrose diet
Background: Increasing prevalence of obesity, overweight and metabolic syndrome secondary to excessive consumption of a high-sucrose and high-fat diet is a major global health problem.
Objective: This study was meant to determine the hypolipidaemic potentials of phytochemicals present inVernonia amygdalina(Bitter leaf) in obese rats.
Method: Thirty-two (6 weeks old, weighing 150-160g) male Wistar rats, were randomly selected and grouped into four: with eight rats in each group. The rats in the control group were fed normal rat chow and water while the three experimental groups were fed the various compounded diets and water ad libitum. At the end of 12th week, the animals fasted overnight, and Oral Glucose Tolerance Test (OGTT) was performed in some while others were anaesthetized using diethyl ether. Fasting plasma, liver tissue lipid and lipoprotein levels were determined, and LDL-C was calculated.
Result: Rapid weight gain (89.9%) was recorded in rats fed High Sucrose Diet [HSD] when compared with the control group (55.3%) without significant differences in the liver weight. The mean plasma glucose, triglyceride, total cholesterol and LDL-C levels were significantly higher (p = 0.000, p = 0.010, p = 0.000 and p = 0.002 respectively) while mean plasma HDL-C was significantly lower (p = 0.043) in rats fed HSD when compared with rats fed normal rat chow. A significantly lower plasma lipid and higher liver tissue HDL-C (p = 0.03) were observed in the Vernonia amygdalina-treated rats.
Conclusion: HSD induces hepatic steatosis and the related biochemical changes while dietary Vernonia amygdalina ameliorates the biochemical changes in hepatic steatosis in rats.
Rocha VZ, Libby P. Obesity, inflammation, and atherosclerosis. Nat Rev Cardiol 2009; 6: 399–409.
Flegal KM, Carroll MD, Ogden CL, Curtin LR. Prevalence and trends in obesity among US adults, 1999–2008. JAMA 2010; 303: 235–241.
Siri-Tarino PW. Effects of diet on high-density lipoprotein cholesterol. Curr Atheroscler Rep 2011; 13: 453–460.
Panchal SK, Brown L. Rodent models for metabolic syndrome research. J Biomed Biotechnol 2011; 2011: 351982.
Ruskovska T, Bernlohr DA. Oxidative stress and protein carbonylation in adipose tissue - implications for insulin resistance and diabetes mellitus. J Proteomics 2013; 92: 323–334.
Rickman JC, Barrett DM, Bruhn CM. Review: Nutritional comparison of fresh, frozen, and canned fruits and vegetables, Part 1: Vitamin C and B and phenolics compounds. J Sci Food Agric 2007; 87: 930-944.
Ojiako OA, Nwanjo HU. Is vernonia amygdalina hepatotoxic or hepatoprotective? Response from biochemical and toxicity studies in rats. Afr J Biotechnol 2006; 5: 1648-1651.
Hakizamungu E, Puyvelde LV, Wery M. Screening of Rwandese medicinal plants for antitrichomonal activity. J Ethnopharmacol 1992; 36: 143-146.
Vlietinck AJ, Van-Hoof L, Totte J, Lasure A, Vanden-Berghe D, Rwangabo PC et al. Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. J Ethnopharmacol 1995; 46: 31-47.
Ademola IO, Eloff JN. The antihelminthic activity of acetone extract and fractions of Vernonia amygdalina against Haemonchus contortus eggs and larvae. Trop Anim Health Prod 2011; 43(2): 521-527.
Tona L, Cimanga RK, Mesia K, Musuamba CT, Bruyne TD, Apers S, et al. In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in the Democratic Republic of Congo. J Ethnopharmacol 2004; 93: 27-32.
Ogbebor NO, Adekunle AT, Enobakhare DA. Inhibition of Colletrotrichum gloeosporioides (Penz) Sac. Causal organism of rubber (Heveabrasiliensis Muell. Arg.) leaf spot using plant extracts. Afr J Biotechnol 2007; 6: 213-218.
Atangwho IJ, Ebong PE, Eyong EU, Eteng MU. Combined administration of extracts of Vernonia amygdalina(Del) and Azadirachta indica (A. Juss) mimic insulin in time-course body weight and glucose regulation in diabetic and non-diabetic rats, Niger. J Biochem Mol Biol 2010; 25(1): 44-49.
Adikwu MU, Uzuegbu DB, Okoye TC, Uzor PF, Adibe MO, Amadi, BV. Antidiabetic effect of combined aqueous Leaf extract of Vernonia amygdalina and Metformin in rats. J Basic Clin Pharm 2010; 1(3): 197-202.
Imaga NOA, Bamigbetan DO. In vivo biochemical assessment of aqueous extracts of Vernonia amygdalina (Bitter leaf). Int J Nutri Metab 2013; 5(2): 22-27.
Keaney Jr. JF. Atherosclerosis: from lesion formation to plaque activation and endothelial dysfunction. Mol Aspects Med 2000; 21(4-5): 99–166.
Shamala S, Baskaran G, Noor AS, Siti AA, Mohd YS. Antiatherosclerotic Effects of Plant Flavonoids. BioMed Res Int 2014; 2014: 1-11.
Kothare PA, Linnebjerg H, Skrivanek Z, Reddy S, Mace K, Pena A, et al.Exenatide effects on statin pharmacokinetics and lipid response. Int J Clin Pharmacol Ther 2007; 45: 114-120.
Gillett RC, Norrell A. Considerations for safe use of statins: liver enzyme abnormalities and muscle toxicity. Am Fam Phy 2011; 83: 711–716.
Pierce LR, Wysowski DK, Gross TP. Myopathy and rhabdomyolysis associated with lovastatin-gemfibrozil combination therapy. J Am Med Assoc 1990; 264(1): 71–75.
Utsumi K, Yoshioka T, Yamanaka N and Nakazawa T. Increase in superoxide dismutase activity concomitant with a decrease in lipid peroxidation of liver during postpartum development. FEBS Lett 1977; 79(1): 1-3.
Kwon S, Kim YJ, Kim MK. Effect of fructose or sucrose feeding with different levels on oral glucose tolerance test in normal and type 2 diabetic rats. Nutr Res Pract 2008; 2(4): 252–258.
Nagele U, Hagele EO, Sauer G. Reagent for enzymatic determination of serum total triglycerides with improved lipolytic efficiency. J Clin Chem Clin Biochem 1984; 22: 165-174.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502.
Schettler G, Nussel E. Colorimetric determination of Cholesterol. Arh Med Soz Med Prav Med 1975; 10: 55.
Sniderman AD, Blank D, Zakarian R, Berjeron J, Frohlich J. Triglyceride and small dense LDL, the twin Achilles heels of the Friedwald formula. Clin Biochem 2003; 36(7): 499–504.
Busserolles J, Rock E, Gueux E, Mazur A, Grolier P, Rayssiguier Y. Short-term consumption of a high-sucrose diet has a pro-oxidant effect in rats. Br J Nutr 2002; 87: 337–342.
Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001; 414: 813–820.
Ogihara T, Asano T, Katagiri H, Sakoda H, Anai M, Shojima N et al. Oxidative stress induces insulin resistance by activating the nuclear factor-kappa B pathway and disrupting normal subcellular distribution of phosphatidylinositol 3-kinase. Diabetol 2004; 47: 794–805.
Hallfrisch J, Cohen L, Reiser S. Effects of feeding rats sucrose in a high-fat diet. J Nutr 1981; 111: 531-536.
Huang B-W, Chiang M-T, Yao M-T, Chiang W. The effect of high-fat and high fructose diet on glucose tolerance and plasma lipid and leptin levels in rats. Diabetes Obes Metab 2004; 6:120.
Srinivasan K, Patole PS, Kaul CL, Ramarao P. Reversal of glucose intolerance by pioglitazone in high fat diet-fed rats. Methods Find Exp Clin Pharmacol 2004; 26: 327.
Peters LP, Teel, RW. Effects of high sucrose diet on body and liver weight and hepatic enzyme content and activity in the rat. In vivo 2003; 17(1):61-65.
Santure M; Pitre M, Marette A, Deshaies Y, Lemieux C, Lariviere R, et al. Induction of insulin resistance by high sucrose feeding does not raise mean arterial blood pressure but impairs haemodynamic responses to insulin in rats. Br J Pharmacol 2002; 137: 185–196.
Kleemann R, Verschuren L, Morrison M et al. Antiinflammatory, anti-proliferative and anti-atherosclerotic effects of quercetin in human in-vitro and in vivo models.” Atherosclerosis 2011; 218 (1): 44–52.
Lee D, Rai C, Songi Y, Sungi K and Kimi J. Effects of Dietary Garlic Extract on Growth, Feed Utilization and Whole Body Composition of Juvenile Sterlet Sturgeon (Acipenserruthenus). Asian-Aust J Anim Sci 2002; 25: 577-583.
Basciano H, Federico L, Adeli H. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab 2005; 2(1): 5.
Angelova P, Boyadjiev N. A review on the models of obesity and metabolic syndrome in rats, TJS 2013; 1: 5–12.
Chen Z, Ichetovkin M, Kurtz M, Zycband E, Kawka D, Woods J, He X, Plump AS and Hailman E. Cholesterol in human atherosclerotic plaque is a marker for underlying disease state and plaque vulnerability. Lipids Health Dis 2010; 9: 61.
Dekker MJ, Su Q, Baker C, Rutledge AC, Adeli K. Fructose: a highly lipogenic nutrient implicated in insulin resistance, hepatic steatosis and the metabolic syndrome. Am J Physiol Endocrinol Metab 2010; 299: E685-E694.
Erasto P, Grierson DS, Afolayan AJ. Evaluation of Antioxidant activity and the fatty acid profile of the leaves of Vernoniaamygdalinagrowing in South Africa. Food Chem 2007; 104: 636-642.
Pang J, Choi Y, Park T. Ilex paraguariensis extract ameliorates obesity induced by high-fat diet: Potential role of AMPK in the visceral adipose tissue. Arch Biochem Biophys 2008; 476: 178–185.
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