In vitro study of the inhibitory potentials of cold and hot aqueous extract of Vernonia amygdalina, Calotropis procera, and Persea americana on α-glucosidase
DOI:
https://doi.org/10.30442/ahr.1002-07-235Abstract
Background: The surge in the incidence of diabetes mellitus has spurred heightened interest on alternative and complementary therapies, particularly those derived from medicinal plants.
Objective: To assess the α-glucosidase inhibitory activity of cold and hot aqueous extracts of Vernonia amygdalina (bitter leaf), Calotropis procera (locally known as “bomubomu” leaves), and Persea americana (also known as avocado pear) to explore their medicinal applicability.
Method: The leaves of V. amygdalina, C. procera, and the seed of P. americana were subjected to cold and hot extraction methods using distilled water. These extracts were specifically targeted for their potential inhibitory effects on α-glucosidase activity, a key enzyme involved in carbohydrate digestion.
Results: The α-glucosidase inhibitory assay showed that the extracts from the plants had maximum inhibitory effects at 20 mg/mL. Cold and hot aqueous extracts of V. amygdalina exhibited maximal inhibitory activities of 100% and 86%, respectively, at 20mg/mL. Cold and hot aqueous extracts of C. procera displayed 100% and 91% inhibitory activities, respectively, at 20mg/mL. Similarly, cold and hot extracts of P. americana exhibited the highest inhibitory activities of 77% and 63%, respectively, at 20mg/mL concentration.
Conclusions: The findings demonstrated that cold and hot aqueous extracts of Vernonia amygdalina, Calotropis procera, and Persea americana exhibited significant inhibitory activities against α-glucosidase. The observed inhibitory effects suggest the therapeutic potential of these plant extracts as natural remedies for managing post-prandial hyperglycaemia in Type 2 Diabetes mellitus.
References
Alhassan AJ, Sule MS, Et-ta’alu AB, Lawal AT. In vitro inhibitory activities of Persea americana seed extracts on α-amylase and α-glucosidase. Bayero J Pure Appl Sci 2017;10:546–552. https://doi.org/10.4314/bajopas.v10i1.103S
Rashid K, Chowdhury S, Ghosh S, Sil PC. Curcumin attenuates oxidative stress induced NFκB mediated inflammation and endoplasmic reticulum dependent apoptosis of splenocytes in diabetes. Biochem Pharmacol 2017;143:140–155. https://doi.org/10.1016/j.bcp.2017.07.009
Fan W. Epidemiology in diabetes mellitus and cardiovascular disease. Cardiovasc Endocrinol 2017;6:8–16. https://doi.org/10.1097/XCE.0000000000000116
Esser N, Paquot N, Scheen AJ. Anti-inflammatory agents to treat or prevent type 2 diabetes, metabolic syndrome and cardiovascular disease. Expert Opin Investig Drugs 2015; 24:283–307. https://doi.org/10.1517/13543784.2015.974804
Kumar K, Rajput R. Current trends of medicinal plants with potential anti-diabetic activity: a review. Paripex-Indian J Res 2018;7:499–503. https://doi.org/10.36106/paripex
Kim KY, Nam KA, Kurihara H, Kim SM. Potent alpha-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 2008;69:2820-2825. https://doi.org/10.1016/j.phytochem.2008.09.007
Terra WR, Ferreira C. Biochemistry of digestion. Comprehensive Molecular Insect Science, Elsevier, Amsterdam, Netherlands 2005.p.171–224. https://doi.org/10.1016/B0-44-451924-6/00053-3
Kumar S, Narwal S, Kumar V, Prakash O. α-Glucosidase inhibitors from plants: a natural approach to treat diabetes. Pharm Rev 2011;5:19. https://doi.org/10.4103/0973-7847.79096
Ghani U. Re-exploring promising α-glucosidase inhibitors for potential development into oral anti-diabetic drugs: finding a needle in the haystack. Eur J Med Chem 2015;103:133–162. https://doi.org/10.1016/j.ejmech.2015.08.043
Djeujo FM, Cusinato F, Ragazzi E, Froldi G. α-Glucosidase and advanced glycation end products inhibition with Vernonia amygdalina root and leaf extracts: new data supporting the anti-diabetic properties. J Pharm Pharmacol 2021;73:1240–1249. https://doi.org/10.1093/jpp/rgab057
Brai B, Adisa RA, Odetola AA. Hepatoprotective properties of aqueous leaf extract of Persea americana mill (Lauraceae) “avocado” against CCl4-induced damage rats. Afr J Trad Compl Alt Med 2014;11:237–244. https://doi.org/10.4314/ajtcam.v11i2.2
Yelne MB, Sharma PC, Dennis TJ. Database on medicinal plants used in Ayurveda. CCRAS, New Delhi 2000;2: 69-73. https://api.semanticscholar.org/CorpusID:70743147
Atangwho IJ, Egbung GE, Ahmad M. Antioxidant versus anti-diabetic properties of leaves from Vernonia amygdalina Del. growing in Malaysia. Food Chem 2013;141:3428–3434. https://doi.org/10.1016/j.foodchem.2013.06.047
Sante DB, Henneh IT, Acheampong DO. Anti-inflammatory, anti-nociceptive and antipyretic activity of young and old leaves of Vernonia amygdalina. Biomed Pharmacother 2019;111:1187–1203. https://doi.org/10.1016/j.foodchem.2013.06.047
Gbolade AA. Inventory of anti-diabetic plants in selected districts of Lagos State, Nigeria. J Ethnopharmacol 2009;121:135–139. https://doi.org/10.1016/j.jep.2008.10.013
Isaac AT, Ganiyu O, Akinyemi AJ, Ajani RA, Olanrewaju BO. Avocado pear fruit and leaves aqueous extracts inhibit α-amylase, α-glucosidase and SNP-induced lipid peroxidation insight into mechanisms involved in the management of type 2 diabetes. Int J Appl Nat Sci 2014;3:21–34. https://www.researchgate.net/publication/264121093
Hiren D. Phytochemical screening and biological activity of Calotropis procera (ait). r. br. (Asclepiadaceae) against selected bacteria and Anopheles stephansi larvae. Int J Plant Res 2011;1:29-33. https://doi.org/10.5923/j.plant.20110101.05
Johnson DB. Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. IndoGlobal J Pharm Sci 2011;1:186-193. https://doi.org/10.35652/IGJPS.2011.18
Kumar JM. In vitro antioxidant and cytotoxic potential of Calotropis procera (r. Br.) Root. IJPSR 2011;2:2132-2135.
Saba AB, Oguntoke PC, Oridupa OA. Anti-Inflammatory and Analgesic Activities of Ethanolic Leaf Extract of Calotropis procera. Afr J Biomed Res 2011;14:203-208.
Hong HC, Li SL, Zhang XQ, Ye WC, Zhang Z. Flavonoids with alpha-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea. Chinese Med 2021;8:1–7. https://doi.org/10.1186/1749-8546-8-19
Ceriello A, Davidson J, Hanefeld M. Post-prandial hyperglycaemia and cardiovascular complications of diabetes: an update. Nutr Metab Cardiovasc Dis 2006;16:453–456. https://doi.org/10.1016/j.numecd.2006.05.006
Mahomoodally MF, Subratty AH, Gurib-Fakim A, Choudhary MI, Nahar KS. Traditional medicinal herbs and food plants have the potential to inhibit key carbohydrate hydrolysing enzymes in vitro and reduce post-prandial blood glucose peaks in vivo. The Sci World J 2012; 285284. https://doi.org/10.1100/2012/285284
Ghosh S, Chowdhury S, Sarkar P, Sil PC. Ameliorative role of ferulic acid against diabetes-associated oxidative stress induced spleen damage. Food Chem Toxicol 2018;118:272–286. https://doi.org/10.1016/j.fct.2018.05.029
Patil P, Mandal S, Tomar SK, Anand S. Food protein-derived bioactive peptides in management of type 2 diabetes. Eur J Nutr 2015;54:863–880. https://doi.org/10.1007/s00394-015-0974-2
Sudha P, Zinjarde SS, Bhargava SY, Kumar, AR. Potent ????-amylase inhibitory activity of Indian Ayurvedic medicinal plants. Evidence-Based Compl Alt Med 2011;1:5. https://doi.org/10.1186/1472-6882-11-5
Mohamed EAH, Siddiqui MJA, Ang LF. Potent ????-glucosidase and ????-amylase inhibitory activities of standardised 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as an anti-diabetic mechanism. BMC Compl Alt Med 2012;12:176. https://doi.org/10.1186/1472-6882-12-176
Oyeyemi IT, Akinlabi AA, Adewumi A, Aleshinloye AO, Oyeyemi OT. Vernonia amygdalina: A folkloric herb with anthelminthic properties. Beni-Suef University J Basic Appl Sci 2018;7:43-49. https://doi.org/10.1016/j.bjbas.2017.07.007
Asante DB, Henneh IT, Acheampong DO, Kyei F, Adokoh CK, Ofori EG, et al. Anti-inflammatory, anti-nociceptive and antipyretic activity of young and old leaves of Vernonia amygdalina. Biomed Pharmacother 2019;1187-1203. https://doi.org/10.1016/j.biopha.2018.12.147.
Erukainure OL, Chukwuma CI, Sanni O, Matsabisa MG, Islam MS. Histochemistry, phenolic content, antioxidant, and anti-diabetic activities of Vernonia amygdalina leaf extract. J Food Biochem 2018;12737. https://doi.org/10.1111/jfbc.12737.
Djeujo FM, Stablum V, Pangrazzi E, Ragazzi E, Froldi G. Luteolin and vernodalol as bioactive compounds of leaf and root Vernonia amygdalina extracts: effects on α-glucosidase, glycation, ROS, cell viability, and in silico ADMET Parameters. Pharm 2023;15:1541. https://doi.org/10.3390/pharmaceutics15051541
Kazeem MI, Mayaki AM, Ogungbe BF, Ojekale AB. In-vitro studies on Calotropis procera leaf extracts as inhibitors of key enzymes linked to diabetes mellitus. Iran J Pharm Res 2016; 15:37–44.
Song Y, Manson JE, Buring JE, Howard D, Liu S. Association of dietary flavonoids with risk of type 2 diabetes and markers of insulin resistance and systemic inflammation in women: A prospective study and cross-sectional analysis. J Am Coll Nutr 2005;24:376–384. https://doi.org/10.1080/07315724.2005.10719488
Ding H, Chin YW, Kinghorn AD, D’Ambrosio SM. Chemopreventive characteristics of avocado fruit. Seminars Cancer Biol 2007;17:386-394. https://doi.org/10.1016/j.semcancer.2007.04.003
Alkhalaf MI, Alansari WS, Ibrahim EA, ELhalwagy MEA. Antioxidant, anti-inflammatory and anti-cancer activities of avocado (Persea americana) fruit and seed extract. J King Saud University - Sci 2019;31. https://doi.org/10.1016/j.jksus.2018.10.010
Lawal TA. Screening of Aqueous Extract of Persea americana Seeds for Alpha-Glucosidase Inhibitors. Hindawi Biochem Res Int 2022; 3492203. https://doi.org/10.1155/2022/3492203
Alhassan AJ, Sule MS, Atiku MK, Wudil AM, Abubakar H. Effects of aqueous avocado pear (Persea americana) seed extract on alloxan-induced diabetes rats. Greener J Med Sci 2012;2:5–11. https://doi.org/10.15580/GJMS.2012.1.GJMS1202
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