Effect of aqueous extract of Allium sativum (Garlic) on fertility in male Wistar rats

Authors

  • AS Faponle Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Sagamu Campus, Sagamu, Ogun State
  • IO Osonuga
  • EN Ezima
  • TK Adenowo
  • AA Adelegan

DOI:

https://doi.org/10.30442/ahr.0601-11-71

Keywords:

Allium sativum, Garlic, Male fertility, Sperm motility, Serum Testosterone

Abstract

Background: Allium sativum (Garlic) is widely used as a food flavour and it has antimicrobial medicinal purposes. It has other beneficial effects such as improving the cardiovascular and immune systems. The likely effect of garlic on the testicular functions remains controversial.

Objective: To investigate the effects of fresh garlic on the fertility of male Wistar rats by quantitative determination of testosterone, follicle-stimulating hormone (FSH), and Luteinizing hormone (LH) levels, as well as the sperm properties.

Methods: The aqueous extracts of garlic were administered to the animals through the oral route. Thirty-five male rats were categorized into seven groups, with five rats each distributed as follows: high-dose, medium-dose, low-dose that were administered 750, 500, and 250 mg/kg body weight dosages of the aqueous extracts, respectively. Others were the control group, which received no extract, and the recovery groups received the same doses of the extract. The recovery groups were left for additional twenty-one days without receiving the extract.

Results: The results showed an increase in testosterone levels at medium to high-doses of the extract including sustained high levels in recovery groups. Also, there were increases in sperm motility and sperm viability.

Conclusions: These findings suggest garlic may play beneficial roles in the reproductive functions of male Wistar rats.  The mechanisms for these effects may involve the interplay of many currently unknown factors.

References

Block E. The chemistry of garlic and onion. Sci Am 1985; 252: 114-119.

Arzanlou M, Bohlooli S. Introducing of green garlic plant as a new source of allicin. Food Chem 2010; 120(1): 179-183.

Block E. Garlic and other Allium: The Lore and the science. 1st Edition. Cambridge: Royal Society of Chemistry. 2010.

Londhe VP, Gavasane AT, Nipate SS, Bandawane DD, Chaudhari PD. Role of garlic (Allium sativum) in various diseases: An overview. J Pharm Res Op 2011; 1(4): 129–134.

Simmoneti, G. Simon. Schuster's Guide to Herbs and Spices (Nature Guide Series). Stanley Schuler (Editor). Fireside; 1990.

Khalil WK, Ahmed KA, Park MH, Kim YT, Park HH, Abdel-Wahhab MA. The inhibitory effects of garlic and Panax ginseng extract standardized with ginsenoside Rg3 on the genotoxicity, biochemical, and histological changes induced by ethylenediaminetetraacetic acid in male rats. Arch Toxicol 2008; 82: 183–195.

Unsal A, Eroglu M, Avci A, Cimentepe E, Guven C, DeryaBalbay M, et al. Protective role of natural antioxidant supplementation on testicular tissue after testicular torsion and detorsion. Scand J Urol Nephrol 2004; 40: 17–22.

Ginter E, Simko V. Garlic (Allium sativum L.) and cardiovascular diseases. Bratisl Lek Listy 2010; 111(8): 452–456.

Brankovic S, Radenkovic M, Kitic D, Veljkovic S, Ivetic V, Pavlovic D, et al. Comparison of the hypotensive and bradycardic activity of ginkgo, garlic, and onion extracts. Clin Exp Hypertens 2011; 33: 95–99.

Rohner A, Ried K, Sobenin IA, Bucher HC, Nordmann AJ. A systematic review and meta-analysis on the effects of garlic preparations on blood pressure in individuals with hypertension. Am J Hypert 2015; 28(3): 414–423.

Jan B, Frank A, Martin CHG, Ifeanyi DN, Alan JS. Allicin: Chemistry and Biological Properties. Molecules 2014; 19(8): 12591–12618.

Ensminger AH, Ensminger ME, Konlande JE, JRK Robson. Food and Nutrition Encylopedia. 2nd Edition. Boca Raton, Florida: CRC Press Inc. 1994: 1318-1319.

Kasuga S, Uda N, Kyo E, Ushijima M, Morihara N, Itakura Y. Pharmacologic activities of aged garlic in comparison with other garlic preparations. J Nutr 2001; 131: 10805-10845.

Ola-Mudathir KF, Suru SM, Fafunso MA, Obioha UE, Faremi TY. Protective roles of onion and garlic extract on cadmium-induced changes in sperm characteristics and testicular oxidative damage in rats. Food Chem Toxicol 2008; 46: 3604–3611.

Sadik NA. Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium-treated male rats. J Biochem Mol Toxicol 2008; 22: 345–353.

Murugavel P, Pari L. Diallyl tetrasulfide modulates the cadmium-induced impairment of membrane-bound enzymes in rats. J Basic Clin Physiol Pharmacol 2007; 18: 37–48.

Dixit VP, Joshi S. Effect of chronic administration of garlic (Allium sativum Linn) on testicular function. Indian J Exp Biol 1982; 20: 534-536.

Hammami I, Nahdi A, Mauduit C, Benahmed M, Amri M. The inhibitory effects on adult male reproductive functions of crude garlic (Allium sativum) feeding. Asian J Androl 2008; 10(4): 593-601.

Hammami I, El May M. Impact of Garlic feeding (Allium sativum) on male fertility. Andrologia 2013; 45(4): 217–224.

Kamenebali I, Aprioku JS. Assessment of the influence of Aqueous Allium sativum Bulb (Garlic) extracts on Testicular Activity in Wistar Albino Rats. Res J Life Sci Bioinfo Pharm Chem Sci 2018; 4(2): 70-80.

Chakrabrati K, Pal S, Bhattacharyya AK. Sperm immobilization activity of Allium sativum L. and other plant extracts. Asian J Androl 2003; 5: 131-135.

NIH. Guide for Care and Use of Laboratory Animals. 11th Edition. National Academics Press, Washington, DC. 2011; 43-48.

Singh UP, Padney VN, Wanger KG, Singh KP. Antifungal activity of aojene, a constituent of garlic (Allium sativum). Can J Bot 1990; 68:1354-1356.

Raji Y, Osonuga IO, Akinsomioye OS, Osonuga OA, Mewoyeka OO. Gonadotoxicity evaluation of oral artemisinin derivative in male rats. J Med Sci 2005; 5(4): 303-306.

Tietz NW (Editor). Clinical Guide to Laboratory Tests, 3rd Ed. Philadelphia: W.B. Saunders. 1995.

World Health Organization. WHO Laboratory Manual for the Examination of Human Semen Sperm Cervical Mucus Interaction Cambridge: Cambridge University Press; 2000.

Norusis MJ. Statistical Packages for Social Sciences (SPSS). SPSS Inc. SPSS/PC+ for Windows, base and advanced statistics users guide, Version 15.0 Chicago, IL: 1998.

Chung LY. The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide. J Med Food 2006; 9(2): 205-213.

Omotoso G, Oyewopo A, Kadir R, Olawuyi S, Jimoh A. Effects of aqueous extract of Allium sativum (garlic) on semen parameters in Wistar rats. Internet J Urol 2010; 7(2): 1-5. Available at https://print.ispub.com/api/0/ispub-article/12374. Accessed on 5th November 2019.

Asadpour R, Shahbazfar AA, Kianifard D, Azari M, Zaboli N. Comparison of the protective effects of garlic (Allium sativum L) extract, vitamin E and N acetyl-cysteine on testis structure and sperm quality in rats treated with lead acetate. Revue Méd Vét 2013; 164(1): 27-33.

Olayiwola OO, Hellevi P, Ilpo TH. Role of Follicle-Stimulating Hormone in Spermatogenesis. Frontiers Endocrinol 2018; 9: 763. https://doi.org/10.3389/fendo.

Salah MMA. Abdul-Lattif RF, Sabrei DA. Physiological and histological effect of aqueous and alcoholic extract of Garlic (Allium sativum) on testicular function of albino male mice treated with lead acetate. J Biotech Res Centr 2014, 8(2): 41-48.

Published

2020-03-28

Issue

Section

Original Research